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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. This patient is most likely suffering from an immediate-acting coagulation inhibitor; most commonly, lupus anticoagulant. Notice that the addition of normal pooled plasma does not correct upon initial or incubated mix, which means that the inhibitor is not time or temperature-dependent.
Factor VIII is not the correct answer as a factor deficiency would have corrected upon the addition of normal pooled plasma. Factor VII is not the correct answer, as the aPTT assay does not account for factor VII activity or concentration.
The laboratorian completed the mixing study ordered for John Doe. The results are as follows:
Initial aPTT result: 167 seconds
Initial 1:1 Mix with Normal Pooled Plasma: 158 seconds
Incubated 1:1 Mix with Normal Pooled Plasma: 150 seconds
Which of the choices below would most likely explain the results for this patient?

A) Time/temperature-dependent coagulation inhibitor
B) Immediate-acting coagulation inhibitor
C) Factor VII deficiency
D) Factor VIII deficiency

2. The steps in the PCR process are:
1. Denaturation (Turning double stranded DNA into single strands.)
2. Annealing/Hybrization (Attachment of primers to the single DNA strands.)
3. Extension (Creating the complementary strand to produce new double stranded DNA.) What is the first step of the PCR reaction?

A) Hybridization
B) Denaturation
C) Annealing
D) Extension

3. Once substance H is developed, the addition of the sugar N-acetylgalactosamine to the terminal position of the chain gives the molecule "A" antigenic activity.
Which specific terminal sugar causes a red cell to have A antigenic activity?

A) N-acetylgalactosamine
B) Galactose
C) Galactose plus glucose
D) Glucose

4. 1+ reaction has numerous small clumps and cloudy red supernatant
2+ has many medium-sized clumps and clear supernatant.
3+ has several large clumps and clear supernatant
4+ has one solid clump, no free cells, and clear supernatant
BB
Tube-based agglutination reactions in blood bank are graded from negative (0) to 4+. A reaction that has numerous small clumps in a cloudy, red background is:

A) 1+
B) 4+
C) 2+
D) 3+

5. 1 SD = 68.3%, 2 SD = 95.5%, 3 SD = 99.7
As defined by a Gaussian distribution curve, what percentage of values would be expected to fall within two standard deviations of the mean:

A) 75%
B) 95%
C) 100%
D) 85%

Solutions: