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American Society of Microbiology ABMM Sample Questions:

1. A patient with HIV infection and a low CD4+ T-cell count presents with fever, weight loss, and cough. Chest X-ray shows diffuse interstitial infiltrates. Sputum induction reveals oval yeast cells with narrow-based budding. The MOST likely diagnosis is:

A) Tuberculosis
B) Cryptococcosis
C) Pneumocystis pneumonia
D) Histoplasmosis

2. The erm gene in Staphylococcus aureus confers resistance to which class(es) of antibiotics via modification of the ribosomal target site?

A) Macrolides, Lincosamides, and Streptogramin B (MLS<sub>B</sub>)
B) Fluoroquinolones
C) Beta-lactams only
D) Aminoglycosides

3. What is the most appropriate method for sterilizing a heat-labile liquid solution containing essential proteins for use in tissue culture?

A) Irradiation with ultraviolet (UV) light
B) Exposure to ethylene oxide gas
C) Filtration through a 0.22 µm pore size membrane filter
D) Autoclaving at 121°C for 15 minutes

4. A patient with a history of recurrent Clostridioides difficile infection (CDI) is being considered for fecal microbiota transplantation (FMT). Pre-FMT screening of the donor stool sample should include comprehensive testing to exclude potential transmission of enteric pathogens. Which of the following would be the MOST critical to rule out in the donor stool?

A) Quantification of the overall microbial diversity using 16S rRNA gene sequencing.
B) Detection of common antibiotic resistance genes not currently expressed.
C) Presence of non-toxigenic C. difficile strains.
D) Evidence of asymptomatic carriage of multidrug-resistant organisms (MDROs) such as vancomycin- resistant enterococci (VRE) or carbapenem-resistant Enterobacterales (CRE).

5. A patient develops severe, watery diarrhea shortly after starting a course of clindamycin. Stool testing using a cytotoxicity assay is positive. This result is most consistent with infection by which organism?

A) Campylobacter jejuni
B) Giardia lamblia
C) Clostridioides difficile
D) Norovirus

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